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KMID : 1041219870290010020
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Journal of Sericultural and Entomological Science
1987 Volume.29 No. 1 p.20 ~ p.30
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Studies on the Vitellogenin in the Silkworm, Bombyx mori, Reared with Mulberry Leaves and Artificial Diet
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Abstract
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In the hemolymph proteins of the silkworm reared with mulberry leaves and artifcial diet, electrophoretic patterns of Storege Protein 1 (SP1) and Vitellogenin(VG), changes of relative concentration of SP1, JHA effects during the developmental stages, and estimation of the molecular weight of vitellin subunits were investigated in the present study.
1. Storage Protein 1 (SP1) showed female specificity from the middle stage of the fifth instar to the end of spinning stage in the silkworm reared with mulberry leaves and artificial diet.
2. Vitellogenin (VG) showed female specificity just after pupation in the silkworm reared with mulberry leaves and artificial diet.
3. Storage Protein 2 (SP2) without female specificity was detected from the early stage of the fifth instar to the first or second day after pupation.
4. Vitellin was composed of two non-identical subunits (vitellin·heavy chain; VTL-H, vitellinlight chain; VTL-L) with molecular weight of 186,000 and 41,000.
Also, there were no differences between the molecular weights of vitellin subunits obtained from silkworms reared with mulberry leaves and artificial diet.
5. Relative concentration of Storage Protein 1 (SP1) after topical application - 0, 5, and 10μg per body weight - of JHA on the 60th hour of the fifth instar showed the highest increase with the treatment of 5μg and a higher increase with the treatment of 10μg compared with the control(no topical application of JHA).
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